A hypothesis or prediction is made with limited evidence at the beginning of a scientific investigation.
Biological knowledge should be used to justify the prediction.
Example
Practical 1.4 - Investigating the effect of temperature on the action of an enzyme
At low temperatures the enzymeProteins that act as biological catalysts, meaning they speed up reactions without being used up themselves. will break starch down slowly due to a lack of kinetic energyEnergy which an object possesses by being in motion. and at very high temperatures starch will not be broken down, as the enzyme will be denatured.